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To score the strength of enhanced intracellular bacterial proliferation (up phenotype), Z-scores above 3 and 2 were considered as strong and medium effects, respectively (see experimental procedures).
Treatment of these cells with resveratrol suppressed cell proliferation (up to 95%) compared to the control.
In parallel, we found that FGF-2 but not FGF18 significantly induced cell proliferation (up to two-fold).
Moreover, pre-incubation of HT-29 cells with IGF-1 for 5 minutes followed by treatment with resveratrol also resulted in suppressed cell proliferation (up to 94%).
Upon exposure of the cells to a combination of VP-16 and 10 μg ml−1 MXF for 48 and 72 h, a marked decrease in cell proliferation (up to 36±0.6 and 45±0.8%, respectively) was observed (P=<0.001).
Interestingly, BRAF mutant melanoma cell lines (UACC903 and Colo829) showed the highest sensitivity to MTA treatment, where concentrations of 10 μM of MTA reduced proliferation up to 70% (Fig 1D).
Similar(34)
We demonstrate that a single dose of anti-CD40 alone can effectively convert CTL exhaustion by activating the mTORC1 pathway, leading to CTL proliferation, up-regulation of an effector-cytokine IFN-γ and the cytolytic effect in AdVova-B6 mice.
It is possible that some of these critical regulators of cell proliferation up-regulated in the trisomy 19 cell lines play an important role in paracrine/autocrine signaling resulting in EGFR overexpression and autoactivation.
In addition to genes with diverse functions that are not directly related to HH-dependent proliferation, up-regulated genes that influence the G1/S transition and subsequent cell cycle progression, and that are common to both cell lines, include CDKN1A, and the DNA-damage-inducible transcripts 3 and 4 (DDIT3 and DDIT4).
Genetic down-regulation of EZH2 by silencing in GM condition reduced RD cell proliferation up-regulating p21Cip1.
Concomitantly, this treatment also led to a reduction in the expression of the cell survival and proliferation up-regulator transcription factor c-myc.
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