Sentence examples for neo expression from inspiring English sources

In fact, selection for Neo expression probably favors the survival of cell lines with integration sites in genomic locations that are transcriptionally active.

Homologous recombination in mouse ES cells transfected with the targeting vector (construction described in the Methods) led to the replacement of Pink1 exons 4 and 5 with a phosphoglycerate kinase (PGK) promoter-driven neomycin phosphotransferase (neo) expression cassette and the deletion of essential portions of the PINK1 kinase domain (Figure 1).

The donor DNA also includes a neo expression cassette to allow selection of successfully targeted cells.

Endogenous APP and NEO expression was also performed using primers directed towards the hAPPsw (see supplementary material Table S1).

Exogenous neo expression showed the same pattern as that of MD-2 and IL-6 but with greater differences between transgenic and wild-type mice.

A neomycin-resistance gene (neo) expression cassette was inserted between the two regions, which resulted in a vector designed to delete the two RNA-binding motifs in an exon 17 and 18.

Murine 4T1 mammary cancer cells (expressing the pcDNA-Neo expression vector as a control; 4T1-Neo cells) implanted into the axillary mammary gland of BALB/c mice metastasized to the mesentery adjacent to the pancreas [ 12].

Bovine peripherin/rds and rom-1 were epitope tagged with an amino-terminal FLAG-tag or amino-terminal hemagglutinin (HA -tag, respectively, and cloned into tHA -tagneo exprespectivelyor for trandient transfeclonedinto COS cells.

For cycloheximide treatment, pCI-neo expression vector that encoding C-terminally-FLAG-tagged Ptc-ICD7 was transfected into HeLa cells.

A targeting vector was designed to replace the open reading frame (ORF) of Slitrk6 with a phosphoglycerol kinase (PGK -neo exPGK -neo cassexpressioned by a loxP sequencassette 3A).

For expression of nematode TRA-2 ICD7 (amino acids 1135 to 1475 of C. elegans TRA-2 protein) [33], corresponding cDNA was subcloned into pCI-neo expression vector with N-terminal FLAG-tag.