Sentence examples for england expression from inspiring English sources

He was long and lean and composed, with an impassive New England expression and a voice that was low but audible, and slightly chilling.

DNA sequences corresponding to the GCK-1 protein fragments in Figure 6A were PCR amplified from a full-length GCK-1 cDNA and subcloned into a pMAL-Maltose Binding Protein (MBP) E. coli expression vector (New England Biolabs (NEB), Beverly, MA).

A PCR construct spanning A.m.H codons N137 to P348 was cloned into pMAL-C expression vector (New England Biolabs).

The cDNA of human ACADM gene (ACADM-encoding pKK223 plasmid obtained as a generous gift from Jerry Vockley, Pittsburgh, USA) was cloned into the pMAL-c2X expression vector (New England Biolabs) encoding a N-terminal MBP-tag and a factor Xa cleavage site.

The England manager usually has a pained expression when asked about an attacker who turns 30 next Monday.

Escherichia coli BLR (DE3) and BL21 (DE3) (New England Biolabs, Beverly, MA, USA) served as expression hosts.

To obtain purified recombinant Mad3p we used the IMPACT-CN expression and purification system (New England Biolabs).

Tagged cDNAs were excised from pCRII TOPO using EcoRI enzyme (New England Biolabs) and cloned into the mammalian expression vector, pCDNA3.1 (Invitrogen).

The assembled fragment was double digested with NheI/ NotI (New England BioLabs) and ligated into the mammalian cell-expression vector pcDNA3.1 (for full sequence see Additional file 2).

The PCR product was ligated into the expression vector using T4 DNA ligase (New England Biolabs).

The fragments were ligated into the PsiI site of the expression cassette (pStLhca3cas) using T4 DNA Ligase (New England BioLabs).