Sentence examples for mutations in endogenous from inspiring English sources

In our present work, we used fibroblast cultures from PD patients carrying two mutated Parkin or PINK1 alleles to investigate the consequences of mutations in endogenous PINK1 and Parkin on the ubiquitination of mitochondrial fusion and fission factors.

Traditional recombination is also capable of creating site-specific mutations in endogenous genes but is laborious and time-consuming.

Recent proof-of-principle studies have shown that ZFNs can also be used to create targeted NHEJ-mediated knockout mutations in endogenous zebrafish genes.

Here, we investigated the impact of mutations in endogenous PINK1 and Parkin on the ubiquitination of mitochondrial fusion and fission factors and the mitochondrial network structure.

These results validate that TALENs induce targeted, predicted loss-of-function mutations in endogenous genomic loci in vivo.

Using this so-called in planta mutagenesis strategy, we were able to recover TALEN-induced mutations in endogenous genes in 1.5 12% of the progeny.

This model contains germline mutations in the endogenous met locus.

We demonstrate that, unlike the wild type protein, misexpression of the ALS-associated VAP mutation has phenotypic effects reminiscent of loss of function mutations in the endogenous gene.

Using this workflow, we detected a variety of TALEN-induced mutations in three endogenous Platynereis genes.

We further examined whether heterodimeric TALENs can introduce mutations in the endogenous gene eyeless (ey).

The result showed that TALEN-702 could induce efficient mutations in the endogenous gene loci through NHEJ repair pathway.