Our method involves isolating and comparing somatic mutations in different subpopulations to trace lineage and infer mutational chronology.
However, no significant difference was found when comparing the frequency of KRAS mutations in different pathology laboratories (data not shown).
Hemophilia was divided into hemophilia A and B, caused by mutations in different genes for different clotting factors.
For research into certain cancers, which can have different mutations in different individual cells, researchers recommend 60 or 80 times.
Mutations in different desmosomal genes have been identified causing dysfunctional cell cell contacts.
This observation shows that activating mutations in different genes of the mTORC1 branch of the mTOR pathway act through convergent mechanisms and have similar phenotypic outcomes.
Comparative genetic and cellular studies provide important insights about the range of mechanisms underlying diversity among vertebrates, including different phenotypic associations with similar mutations in different species.
In some cases even mutations in different genes can lead to the same clinical disorder (genetic heterogeneity).
Different cell lines had mutations in different genes, but a disproportionate number of the mutations appeared in genes involved in cell growth or in genes that have been previously linked to cancer.
We therefore assumed that off-target hits by Cas9 will not independently generate the same de novo mutations in different single-cell derived clones and only considered mutations that occur in each individual sample.
(a) Basal ROS levels in 3 healthy control cell lines (C1 to C3) and 7 patient cell lines (P1 to P7) bearing mutations in different nuclear encoded Complex I subunits.
