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EGFR mutation status of all samples was assessed using a Scorpion Amplification Refractory Mutation System (ARMS -based EGFR mutARMS -basedtion kit (ThEGFRcreen EGFR RGQ PCR kit, Qiagen, Crawley, UK), which detects 29 mutations across the EGFR gene.

Cycling conditions were 94°C for 2 min and 30 cycles of 94°C for 30 sec, 55°C for 30 sec, 72°C for 2 min, followed by 74°C for 5 min. Products were denatured and reannealed, treated with the Surveyor Mutation Detection Kit for WAVE Systems (Transgenomic, Inc ., and injected on the dHPLC WAVE System (Transgenomic, Inc).

Then, SPACE-HRM, direct DNA sequencing, and Scorpion-ARMS simultaneously diagnosed these samples with ADX- EGFR/ KARS mutation detection kit (Amoy Diagnostics Co. Ltd., Xiamen, China).

Samples were amplified using polymerase chain reaction (PCR) and the Surveyor Mutation Detection Kit (Transgenomic, Omaha, NE, USA) was used to screen for p53 mutations.

The tumor tissue was analyzed for the presence of an EGFR mutation using the PNAClamp™ EGFR Mutation Detection kit (PANAGENE, INC. Daejeon, Korea).

Three trials evaluated EGFR mutation status using a range of methods (EGFR mutation detection kit [DxS, Manchester, UK], and sequencing of polymerase chain reaction products from exons 18 to 21 of the EGFR gene).

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Many KRAS exon 2 mutation detection kits such as TheraScreen Kit are approved as IVD in Japan, USA, and Europe.

In a blinded sub-study of 100 CRC samples, we compared the two CE-marked mutation detection kits, PyroMark KRAS and TheraScreen K-RAS mutation kit, by adhering to the manufacturers' protocols and guidelines for interpretation of the results.

Peptide nucleic acid-locked nucleic acid (PNA LNA) PCR clamp reactions were carried out using the PNA-Clamp™ BRAF, and PNA-Clamp™ PIK3CA Mutation Detection Kits (Panagene, Inc., Daejeon, Korea), as described previously.

It is hard for imaging examination to distinguish the real invasion or tumor just leaning against adjacent organs or tissues; for effective neoadjuvant therapy, aspiration biopsy is necessary for not only diagnosis but also gene mutation detection for KIT and PDGFR, but conducting biopsies in such highly malignant GIST may increase the risks of tumor rupture or implantation metastasis.

10 A multiplex mutation detecting kit using Luminex technology (Mebgen Rasket Kit; Medical and Biological Laboratories, Nagoya, Japan) has been approved for the simultaneous detection of 48 types of RAS mutations.

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