Sentence examples for mutation detection assay from inspiring English sources

A rapid and reliable mutation detection assay has been developed.

Having validated our ZFN expression vectors and mutation detection assay, we next tested each of the five ZFN pairs we made by OPEN in zebrafish embryos.

Amplicons were analyzed for mutation by Surveyor Cel-1 enzymatic mutation detection assay (Transgenomics, Omaha, NE).

These data validate the function of assembled TALENs using Surveyor nuclease mutation detection assay.

The cleavage efficiency was measured using the T7 endonuclease I (T7E1) mutation detection assay.

Finally, we measured the sensitivity of our mutation detection assay by performing a dilution of mutation template into increasing amounts of wild-type genomic DNA lacking the mutation.

The aim of this study is to assess the feasibility of TaqMan Mutation Detection Assays (TMDA) based on castPCR technology to detect EGFR mutations in NSCLC clinical specimens and to compare this method with routine diagnostic techniques including direct sequencing and the EGFR Therascreen RGQ kit.

We designed EGFR mutation detection assays by combining allele-specific PCR amplification with the detection of SYBR Green I fluorescence, and optimized PCR conditions to specifically amplify mutant alleles.

A variety of PCR methods, increasingly real time PCR (Q-PCR) targeted mutation detection assays, are currently applied for diagnostic KRAS mutation assessments [20], [21], some of which have already acquired the license for in vitro diagnostic (IVD) use in Europe [22].

There are no Federal Drug Administration-approved KRAS mutation detection assays, and the European Medicines Agency does not recommend any particular methodology (Bellon et al, 2011).

Our T7E1 mutation detection assays confirmed that the use of this CRISPR target did not introduce significant off-target cleavages.