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It showed that in addition to a single distal histidine with a suitable distance to the heme iron (Phe43 to His43 mutation), a second distal histidine (Leu29 to His29 mutation) can work cooperatively to increase the turnover number, mimicking the role of well-known His-Arg pair in native peroxidases.

If the patient's results show an inherited mutation, a second visit is typically scheduled to discuss screening and management options as well as risk to other family members.

In all sheep exhibiting the c.2746delCA mutation, a second intronic c.2749+51A>G mutation could be shown.

Given one OMIDI mutation, a second random mutation 3' to the first (either IMIDI or OMIDI) will restore reading frame only 1/3 of the time, and a sub-fraction of that third is still protein truncating because a nonsense mutation occurs prior to the second mutation.

When they then evaluated which mutations were shared in a single cell, they identified one population with only an ATM mutation, a second with ATM and FSIP2 and a third with ATM, CLTC and GLMN mutations.

To ensure that the altered responses to the pathogen were caused by disruption of the PAP5 gene and not by an unlinked mutation, a second knockout mutant line salk_081481C (pap5-2), carrying a T-DNA insertion on PAP5 (At1g52940), was tested.

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Similar(53)

I then mutated one individual with one mutation, mutated a second individual with the second mutation, and mutated the third individual with both mutations, creating both single mutants and a double mutant.

For specific sub-group comparisons of all ALS cases (FALS/SALS, SOD1 mutation/non-SOD1 mutation) a third set (set III) was constructed based on set I and set II.

Compensatory mutation: A second-site mutation that occurs after a mutation that confers resistance, which lessens or alleviates the fitness costs associated with resistance.

Heterozygous carriers of the 985A>G mutation without a second mutation (cases 20, 22 and 23) had residual MCAD activities of 24% or higher (Table 1).

For double mutations, a second round of site-directed mutagenesis was performed utilizing pRSFDuet-1nisAB-M21P as the plasmid template for the generation of NisA N20P/M21P and M21P/K22P mutants.

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