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The protein of interest is fused through its C-terminus, to the N-terminus of an intein, in which the C-terminal Asn has been mutated to be incapable of cleaving the C-terminal binding tag.
In addition, re-expression of a kinase-dead EGFR, mutated to be unrecognized by shRNA #4, was able in part to recover the viability of SUM102 cells treated with HGF.
A construct was generated in which the conserved loop region, containing the extra base-pairing sequence, was mutated to be complementary to the sequence immediately upstream of the new target site.
Simulations were performed in which each new cell inherited the h value of its parent, but with a small probability, the offspring h was mutated to be slightly higher or lower than the parent.
Therefore, if the snoRNA is mutated to modify a new target site, the conserved region should only enhance methylation when it is mutated to be complementary to the region adjacent to the new target site.
One is to take advantage of the natural coupling between binding and folding using proteins that are either intrinsically disordered in the absence of ligand or mutated to be so.
Similar(54)
For its part Bild, according to the media analyst website Meedia, "is suddenly mutating to be sympathetic towards women.
We've been warned multiple times about how diseases are mutating to be more resistant to antibiotics but we're still struggling to treat drug-resistant TB patients.
All legends have a tendency to mutate, to be reshaped in each successive era according to the prejudices and preoccupations of those who retell the tale.
Our results indicate that these viruses could mutate to be transmissible from birds to mammals, including humans.
A triple mutant where all three histidines were mutated to alanine was constructed by introducing the H306A mutation into the H252A/H232A double mutant.
Related(20)
relocated to be
promoted to be
retired to be
reassigned to be
transferred to be
deployed to be
assigned to be
posted to be
shifted to be
turned to be
moved to be
mutated to create
mutated to contain
mutated to nestle
mutated to generate
mutated to leucine
mutated to have
mutated to cause
mutated to become
mutated to respond
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