Sentence examples for mutated to generate from inspiring English sources
Further, in prior publications, we have shown that targets downstream of 185delAG, including maspin, are unaffected when 185delAG is itself mutated to generate a further BRCA1 mutation [ 39] suggesting that the 185delAG BRCA1 mutation may promote tumorigenesis by a unique mechanism.
C316S and L248V were further mutated to generate C316A and L248A with the associated primers (Tables S2 and S3, Supporting Information).
To determine the effect of the four residues previously implicated in urate binding to HucR and PecS, the equivalent residues in MftR were therefore mutated to generate W11F, D56S, R63S, and R89N MftR variants.
The EXO1 gene was then mutated to generate the desired codon changes corresponding to the following mutations S372A, S372E, S567A, S567E, S587A, S587E, S692A and S692E.
The sites were sequentially mutated to generate Flag-G9a4KR, where all four acceptor lysines were changed to arginine.
The current-sol is mutated to generate a new solution called new-sol.
Furthermore, in microorganisms such as yeast and fungi, the mutator system has been employed to generate various mutated phenotypes including drug resistance.
Alanine scanning mutagenesis of hVβ2.1 wild-type and mutated residues allowed us to generate a map of the binding site for TSST-1 and to construct a docking model for the hVβ2.1 TSST-1 complex.
We used PCR stitching and isothermal assembly to generate mutated versions of these constructs.
The primers used for mutagenesis were: KF385 5'-AGCATTGTTAGTAATCCGCTGC TTTTTATTTA theCACTGGCACCATCACCTTGCG-3' (the mutated nucleotides are underlined) to generate mTRE1, KF386 5'-CCCTCAGACTTCAGGCTAAGG AACCATATGA AAACACCCCGGGAtoATTTATT-3' to generate mTRE2.
Three of these parameters with binary values were first used to construct 8 setting profiles (Table 1).> Mutation rate is the probability for any base to be altered by an SNV or indel from the reference genome, before the resulting mutated genome was used to generate artificial sequencing reads.
