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Proteins can be labeled on native amino acids or mutated to contain a site-specific amino acid.
This copy was mutated to contain various point mutations.
All three catalytic subunits of Protein Kinase A (PKA) were mutated to contain an analogue-sensitive M→G mutation (TPK1 M164G TPK1 M147G TPK1 M165G).
FL human FUS/TLS cDNA (NM_004960) (WT-FUS) was mutated to contain either one of the five clinical mutations (R514G, R521G, R522G, R524S and P525L).
However, when the contact residues in KIR2DS2 were mutated to contain these of KIR2DL2 (KYK/KFK) efficient binding to HLA-G was observed (Figure 3).
The pNL4-3 HIV plasmid had been mutated to contain unique restriction enzyme (BstEII) sites at the 5' end of the gag gene and 45 nucleotides downstream from the 3' end of the protease gene, as described elsewhere [19].
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We expect the mutated sites to contain ~975 neutral sites and 25 selected sites, while the conserved sites should contain ~950 neutral sites and 50 selected sites.
Mutated plasmids were constructed to contain two mutated seed sequences for miR-26a and miR-30c (from ACTTGA to GACGTC for the miR-26a binding site and from TGTTTAC to GACGAGT for the miR-30c binding site).
Seven of the 13 patients with p53 mutations were shown to contain multiple mutated tissue samples.
80% of BRAF-mutated melanomas were found to contain a V600E substitution, which is thought to constitutively activate the kinase by mimicking phosphorylation [1].
Women (have two X-chromosomes) on the other hand, need both copies of their X-chromosomes to contain the mutated gene to be affected by the disease.
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CEO of Professional Science Editing for Scientists @ prosciediting.com