Sentence examples for mutated the first from inspiring English sources

To test whether this preference is maintained for the U2AF35-sensitive cr3′ss+81, we mutated the first exon positions systematically and examined splicing of the mutated constructs in U2AF35-depleted cells.

To engineer an orange derivative of DsRed-Express2, we mutated the first residue of the chromophore, glutamine-66, to threonine.

To further characterise the dCPAP binding domain in vivo we mutated the first proline and arginine of the PRxxPxP motif of dSTIL to alanine, both separately and in combination (P11A, R12A, and P11A R12A, Figure 2C).

To prevent this, we mutated the first 5 amino acids to a sequence that is predicted to be N-terminally acetylated with an 83% likelihood of producing acetylated Ngn3 (AcNgn3).

We mutated the first NotI site (NotI820) of pCMV- β (Clontech) to a KpnI site using following primers : forward 5'-GCT-GCG-GAA-TGG-TAC-CCG-CGG-CCG-CAA-TTC-3' and reverse 5'-GAA-TTG-CGG-CCG-CGG-GTA-CCA-TTC-CGC-AGC-3'.

This interaction is consistent with the findings of Liu et al, 48 who mutated the first four residues from extracellular loops of both alpha and beta1 by changing each one to a cysteine and thus found a close relationship between S1 and TM1.

I then mutated one individual with one mutation, mutated a second individual with the second mutation, and mutated the third individual with both mutations, creating both single mutants and a double mutant.

We mutated the second allele of sik1 by cultivating heterozygous mutant cells (sik1wt/flp) in increasing concentrations of the neomycin analogue G418, as previously described [15].