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Exact(6)
No significant difference in this parameter was documented between the ACHE mutants, with both age groups being similar to young wild-type zebrafish.
Drosophila mutants with both loss-of-function or overexpression of nebula exhibit severe learning defects that are attributed by biochemical perturbations rather than maldevelopment of the brain [12] and the Rcan1 knockout mice present pronounced spatial learning and memory deficits in the Morris water maze task and impaired long-term potentiation (LTP) [13].
(B ) Fraction of CG trajectories that reach fully integrated topologies as a function of time for the EmrE and CB mutants with both loop lengths.
Incubation of all the mutants with both PNGase F and EndoH enhanced the formation of high-molecular-weight species on the top of the gel.
These mutants can be used as controls to further confirm the energy transfer observed in the mutants with both Trps in the same domain.
It should be noted that introduction of the GFP-tagged versions of several RNA polymerase I and III subunits into the GPN mutant background resulted in lethality, suggesting genetic interactions of GPN mutants with both RNAPI and III; indeed, this is why the gpn2 -4 allele was used instead of gpn2 -2 (data not shown).
Similar(54)
The single mutant C320S and a double mutant with both cysteines replaced by serine have been compared with one another in terms of long-term stability and other properties.
eDouble mutant with both mutations at nucleotide positions 165 and 171.
To produce the mutant with both binding sites deleted, a Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, USA) was used with an annealing temperature of 72 °C.
The resulting pRad-155 mutants with one or both Egr1 binding sites mutated were designated pRad-155m1, pRad-155m2 pRad-155m355m3.
Nonetheless, we tested mutants with interruptions in both the sadB [19] and lasI [39] genes that have been shown to affect biofilm formation in dynamic flow cell assays and found they were also not attenuated in our in vivo system.
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