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By testing individually selected clones, we confirmed that growth selections enriched for clones with both improved ethanol tolerance and production phenotypes.
Thus, DPWP demonstrates high discriminative power to identify clones with both drought tolerance and improved yielding ability, especially in root crops such as sweetpotato, which occasionally produce pencil roots instead of edible storage roots even under adequate moisture conditions.
The clones with both inserts were selected on Luria-Bertani (LB) agar containing 100 μg/ml of ampicillin.
Our goal was to maximize the number of clones with both ends sequenced (paired BES) to increase coverage and the chances to capture all inversion breakpoints.
Of the clones with both mate-pair BES available, 6,623 clones (15.9%) yielded significant mappings at the same E-value threshold as before.
The highest expressing clones with both constructs had a copy number of three, but so did some of the lowest expressing clones for both constructs.
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However, this is not actually a solution because the later mutation leads to a fitness disadvantage for the metastatic clone and that clone with both mutations should not expand, which mirrors the original framing of the problem.
A clone with both properties served as a donor for a second round of transduction, in order to estimate the physical distance between its marker and the beneficial mutation of interest.
The data provide evidence of an increased apoptotic fraction (arrowed) in four independent PNT1a-clu clones compared with both PNT1a-hyg clones and PNT1a parental cells.
Clones with knockout for both alleles of each gene were selected by culturing transfected cells with hygromycin and neomycin and initially confirmed by immunoblotting (Figure 3A).
The three cases with complex karyotypes (6, 7 and 8B) all exhibited several related clones with variability in both structural and numerical aberrations.
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