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They found that functional alteration of Hsp90 resulted in transposon activation and the induction of morphological mutants which indicated that Hsp90 mutation or inhibition can generate new genetic variation by transposon-mediated mutagenesis [ 48].
Correlation co-efficiency was calculated between the two biological replicates in each condition (r =0.96 in wild type; r =0.99 in tfc6 mutants), which indicated a high correlation between our biological replicates.
However, SA inducible genes PR1 and PR5 were slightly induced at 1 dpi in overexpression lines as compared with wild type (Col) and mutants which indicated that the initial responses of the plant might be regulated by SA.
Also, deletion of the gene that encodes Spot 42, i.e., spf (spot fourty-two), resulted in viable spf null mutants, which indicated that the Spot 42 RNA is non-essential [ 6].
A double mutant of hst-3.1 and hst-3.2 did not show enhancement of the phenotype when compared with either of the single mutants, which indicated that both HS 3- O-sulfotransferases are acting within the same genetic pathway and serve nonredundant functions to regulate HSN axonal branching.
Similar(55)
The total cell protein profile (Figure 7B) was comparable in the wild type as well as C-terminal and N-terminal mutants which indicates that the expression of EvpC was unaffected by these mutations.
At the same time, we did not observe any aggregate formation of protein samples when tested using DSC except for ΔW6 and ΔY343 mutants, which indicates that decreased mobility of heated protein samples could be due to the unfolding of protein molecules rather than protein aggregation (Figure S2).
We found that β4GalNAcTA mutation semi-dominantly suppressed the phenotype of DSiaT mutants, which indicates that the DSiaT phenotype is very sensitive to the level of β4GalNAcTA activity.
The expression profile of these genes remained unaltered at 9 dpi in quadruple-DELLA mutants infected with TMV-Cg compared to the non-infected quadruple-DELLA mutants; which indicates a clear role of DELLA in tested genes alteration.
The increase of LEC number in Nrp2 taugfp/+;Sema3f +/− double heterozygous mutants was as much as that in Sema3f −/− homozygous mutants which indicate a clear genetic interaction in the LEC number between Sema3f and Nrp2.
Moreover, we calculated an ADP binding stoichiometry of 4 for both wild type and mutants, which indicates that although ATP hydrolysis is strongly affected, detachment of the MD does not change the nucleotide binding.
Related(14)
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