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In S. pombe, the Sipiczki laboratory isolated a number of caffeine-resistant mutants which defined single loci.
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The probability of resistance is the same as the probability to generate mutants, which is defined by the number of cell divisions (and the constant mutation rate).
This is also the case in distorted mutants [ 52], which define genes coding actin polymerization regulating proteins, like components of ARP2/3 [ 29, 30] and SCAR/WAVE complexes [ 31- 34].
Here we show that the proAtHB8∶GUS marker is very poorly localized in the hyd mutants, particularly in embryos where longitudinal procambial traces are established which define the plant body, and also in cotyledons and true leaves of establishment-stage seedlings.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com