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Notably, these animals are not as severely affected as gtl-1 single mutants, which contain only approximately 0.4 times as much Mg2+ as wild type [9].
In this study, we have shown that plants infected with C4 deficient BSCTV mutants, which contain premature C4 but not alter the amino acid sequence of the overlapping Rep protein, developed asymptomatic phenotype (Fig. 1).
In this study, unc-108/rab-2 trafficking mutants, which contain mislocalized GLR-1 in large accretions in the ventral cord (similar to lin-10), also accumulated GLR-1 in Synxtaxin-13-labeled endosomes in the cell body.
In addition, according to the data from the cell protection and SPR assays, both M1 and M2 mutants, which contain the L56A mutation, had a striking increase in protective ability and PA binding affinity, further supporting the important role of position 56 in determining PA-binding affinity.
To further determine the type of polyubiquitin linkage with which Tat is modified, we constructed ubiquitin-K29, -K48, and -K63 mutants which contain only a single lysine (K29, K48, and K63, respectively) with all the other lysines mutated to arginine.
To rule out the possibility that abnormal motor behavior itself alters free amino acid levels, we examined beo mutants, which contain a mutation in the glycine receptor β2 subunit and exhibit abnormal behavior similar to que mutants (Fig. 5C) (Hirata et al., 2005).
Similar(54)
Therefore, we investigated one of our CTD mutants, which contains regular insertions of 5 alanines between CTD diheptapeptides, hereafter referred to as the "5A mutant" (see Fig. 1).
Phytochelatins were absent under control conditions in roots, except in vtc1-1 mutants, which contained a low concentration of PC2 (i.e. 14·75 nmol GSH equivalents g 1 f. wt) (Fig. 5).
By contrast, the growth of all JmjC-demethylase deletion mutants – which contained URA3 reporters integrated into the HMR or HML loci – was indistinguishable from that of an isogenic WT strain (Additional file 1: Figure S1).
Conversely, in nodules infected by the S. meliloti bacA mutant which contain symbiotic cells with undifferentiated bacteroids, only a subset of NCR genes is activated while in other mutants, forming normal symbiotic cells with differentiated bacteroids, NCR genes are activated to a similar extend as in the wild type [ 18, 19].
On fibronectin-coated substrata, this response can be abrogated by culturing cells on rigid substrata coated with a fibronectin mutant which contains a stabilized RGD and PHSRN synergy site that supports α3 and α5 integrin engagement [156].
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