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One approach to unraveling the complexities of the POMC system is to engineer mouse mutants which lack specific POMC peptides.
To this end, we used fliGHI mutants which lack expression of both S. Typhimurium flagellins, fliC and fljB.
However, the egr66 mutants which lack the entire egr coding region behave like wild-type controls (Figure 4C).
Therefore, there is a need for methodologies that allow for the generation of mutants which lack resistance markers and permit successive gene deletions within the same genome.
Of all the mutants tested, only pept-1 mutants, which lack a functional intestinal peptide transporter, fail to show differential fat stores.
However, hyperfilamentous mutants which lack the hyphal repressors Nrg1 or Tup1 and which cannot grow in the yeast form are also reduced in virulence [20], [21].
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The wild type CrSPI-1-D1 and the mutants which lacked thrombin inhibition did not show any binding with thrombin.
By immunoblotting the pattern of all GFP-β2-syntrophin S/D mutants was similar to that of GFP-β2-syntrophin, except for the S75D mutants, which lacked one species (Fig. 5B and Fig. S6B).
We also compared in vitro CMV genomic RNA reassortants, and CMV RNA 3 mutants which lacked the C' terminal 33 amino acids of the 3A movement protein in attempts to further increase E1 yield.
Another study by Palmeira and co-workers [ 50] demonstrated the application of recombinant AlHV-1 ORF73 null mutants, which lacked expression of the latency-associated nuclear antigen (LANA) homolog encoded by ORF73, as a promising recombinant vaccine candidate.
The map shows that the constitutively active mutant, which lacks the C-terminal domain involved in osmosensing, is trimeric like wild-type BetP.
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