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In contrast, the GST activity of 124-site mutants shows a sequential mechanism, as is observed for the native GPx activity of Ure2p, and typical GST enzymes.
The third class of mutants shows a defect in membrane tubulation activity.
Further, none of the secondary scaffoldin mutants shows a significant decrease in the concentration of cellulosomes on the cell surface.
Carboxypeptidase E removes C-terminal lysine and arginine residues from pro-peptides during maturation, and analysis of peptide extracts from egl-21 mutants shows a deficit in the maturation of most C. elegans neuropeptides (Husson et al., 2007).
Scale bar 30 μm. (D and E ) Quantitative analysis of dendrite-ECM interaction and dendrite crossing in wildtype and rac1 mutants shows a significant increase in dendrite detachment (C ) and Z crossing points (D ) in rac1 deficient C4da neurons.
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Rice plastochron (pla) mutants show a short plastochron and small precocious leaves.
Four mutants show a higher reaction rate with the radical compared to the wild type.
Furthermore, all mutants showed a significant 40 °C increase in optimum temperature compared to wild-type enzyme.
The rice mutants lacking AS1 (as1 mutants) showed a decrease in asparagine content in the basal portions of shoots.
Rice mutants lacking GS1 2 (gs1;2 mutants) showed a remarkable reduction in the contents of both glutamine and asparagine in the basal portions of shoots.
Mutant M5A and all three deletion mutants showed a dramatic loss of luciferase activity (Figure 4B).
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