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CLASP is a key regulator of microtubule (MT) dynamics and bipolar mitotic spindle structure with CLASP mutants displaying a distinctive monopolar spindle phenotype.
The structural dynamical properties of DNA in complex with native topoisomerase or with single mutants, displaying a varied reactivity toward CPT, have been extensively investigated by Molecular Dynamics (MD) simulations, permitting to observe a direct correlation between functional properties and inter-domain communication [14] [19].
Our hypothesis predicts that in the absence of whirlin, the whirlin/p55/4.1R/gelsolin complex would not be formed and that the whirler mutant would be epistatic to gelsolin, with double mutants displaying a shortened stereocilia phenotype and an absence of the elongated stereocilia phenotype displayed in gelsolin mutants.
Only mutants displaying a ≥ 100-fold differential in titer compared with production of virus from insect cells were considered to be HR mutants (Table 1).
When grown under light without exogenous sucrose, mutants displaying a constitutive ethylene response phenotype have small and compact cotyledons and the seedling hypocotyl and primary root are shorter than wild type [ 11].
When the two second-shell ligands, D181 and S230, were subjected to pairwise saturation mutagenesis, a large number of unique mutants displaying a significant amount of activity were found.
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The spl28 mutants displayed a necrotic lesion phenotype spontaneously in the absence of pathogen attack.
Kit mutants displayed a lower intrinsic kinase activity relative to PDGFRA mutants, while the KIT Exon 11 deletion mutant exhibited the highest intrinsic kinase activity among KIT mutants.
Substitution of alanine for Arg93 or Arg175 induced a 7 8-fold decrease in inhibition potency, while Arg165Ala, Lys169Ala, Arg17 8-folddecreaseAla thrombin mutants dinhibition 2–4-fold decrease.
Dg mutants displayed a strongly reduced Dys staining (Fig. 3B1-3).
Double pop2-5 ssadh-2 mutants displayed a phenotype characteristic of pop2 mutants (Figure S1).
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