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These mutants are located in close proximity to, and share similar properties with E2-G60D; they both decrease the net positive charge of the E2 protein, indicating that their mechanism of action in SINV and CHIKV may be the same.

In contrast to P15 and P16 mutants, the P4, P17 and P20 mutants are located on the same side of the G α-helix that faces the APE-loop (see below).

The inactive RFM4 and RFM10 mutants are located in the N- and C-terminal boundaries of a large predicted disordered region extending from amino acid ∼100 to amino acid ∼400 (Figure 7B).

Notably, the insert in H522 is in the small globular Domain 2 of the EspP model, whereas the neighbouring mutants are located in the adjacent β-helix (Fig. 4A).

Although the I424S and previously characterised G425R mutants are located in close proximity within the UBA tertiary fold they exert distinct effects on protein function and structure.

The missense mutation p.Ala274Val and p.Phe80Ile found in this study have not been reported yet, and these mutants are located in the transmembrane helix structure of G6PC.

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PRL-3-WT and PRL-3-C171S PRL-3-C171S PRL-3-C171S cytoplasmutantsbrane (Fig. 2).

Genes with a significant change in expression in all three mutants were located on the chromosome, with four exceptions, among them the luxI 3 gene.

As expected, a large portion of amino acid substitutions of the selected dnaQ mutants were located in or quite close to these three regions, meaning high possibilities to disturb the natural proofreading function of the respective gene products.

However, as the area of impaired outflow tract cushion closure in Robo1/2 double mutants was located in the neural crest derived part of the cushion, this still indicates a possible other role for the neural crest in the development of the membranous septum defects.

Up to 5283 (46%%) from the 11,508 IPACs in oxt6 mutant are located within 50 nt of IPACs in WT, indicating that about half of poly(A) sites in intergenic regions are shared by both WT and oxt6 mutant.

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