Sentence examples for mutant were incubated from inspiring English sources

(a) S. aureus RN6390 and the isogenic UHPT-deficient mutant were incubated with either [18F]FDG or [18F]FDG-6-P.

Briefly, wild-type TEM-1 and the V216C mutant were incubated with fluorescein-5-maleimide and then purified by dialysis to remove excess fluorophore.

To prove that ERK kinase directly phosphorylates FBW7, glutathione S-transferase (GST -fused recombinant FBW7 proteins, both wild type and T205A mutant, were incubated with purified HA-ERK1 kinase in the presence of ATP and detected with the p-FBW7 antibody.

Samples containing 1.5 μg of Arfrp1-His, Arfrp1-FLAG or Arf1-FLAG in the presence or absence of 1 μg MBP-Vangl2 cytosolic domain wild type or tyrosine mutant were incubated with 8 μl of 1.8 mg/ml liposomes in HKM buffer containing 100 μM nucleotides at room temperature for 45 min in 50 μl of reaction mixture.

In addition, IM-sensitive GIST-T1 (exon 11-mutant) cells and the highly IM-resistant subline GIST-T1-juke (exon 11 and exon 17 doubledouble mutant) were incubated in vitro with panobinostat at concentrations that were achieved in study patients as confirmed by our PK analyses.

Furthermore, when wild-type HA-cytSec61β but not the Δ20 44 mutant, was incubated with tubulins, anti-HA antibodies precipitated tubulins (Fig. 2G).

10 µg of His-tagged integrase or its deletion mutant was incubated with 10 µg of SIP1 and 30 µl of Ni-NTA agarose (QIAGEN) in storage buffer (20 mM HEPES pH 7.5, 0.1 mM EDTA, 300 mM NaCl, 10 mM chaps, and 20% glycerol) at 4°C.

Heat-sensitive oligomers were observed when trypsin-activated F371A mutant was incubated with BBMVs.

To investigate the stability of PL11, the mutant was incubated in the absence of pleuromutilin.

In this latter experiment, the C-terminal Cys497-Sec498 redyaddyad was mutated to a Ser497-Cys498 dyand and this TR mutant was incubated with the C35S/C73S mutant of hTrx1.

In contrast, a prominent population of microcapsules with increased optical density was detected when the auxotrophic mutant was incubated in the presence of uracil and uridine.