Sentence examples for mutant were treated from inspiring English sources

To assess whether EGF mediated AKT activation is dependent upon Tyr176-phosphorylation, MEF1&2KO cells expressing AKT or Y176F mutant were treated with EGF ligand.

Recombinant proteins of wild Nm23-H1 and C109A mutant were treated with 2.5 mM oxidized glutathione (GSSG) or 5 mM reduced glutathione (GSH) for 1 h at 37°C.

When cells expressing this mutant were treated with LMB, the 32 kDa band still appeared.

All RNA samples of the wild-type strain and D hfq mutant were treated similarly throughout the experimental steps.

Embryos of the tremblor mutant were treated with a wide variety of chemical compounds with the aim of finding some that could correct the heart defect.

(J ) YFP-LC3 and mCherry-Parkin stably expressing TBC1D15−/− cells in the presence of HA-tagged TBC1D15 WT or F280A mutant were treated with valinomycin for 3 hr.

Several crucial PHB biosynthesis genes were deleted through homologous recombination, then the PHB-deficient mutant was treated with plasma mutagenesis to obtain NXdP, an engineering strain.

Fc2 (5 μg) or the alanine mutant was treated with FGE as stated above.

Each set of measurements (those of T50 and [urea]50) was performed on all of the mutants in the same day, and each mutant was treated identically.

For microarray experiments, mid-log phase grown fur mutant was treated with iron chelator for one hour and then ferrous sulfate.

Thus, we generated double mutant GFP S147C, T230C, 233Δ) 8. Initially, to explore if there was any difference in the nucleophilicity of the cysteine thiols at positions 147 and 230, the double mutant was treated with a stoichiometric amount of N-methylmaleimide.