Sentence examples for mutant was subjected from inspiring English sources

To confirm that the deletion of FTL_1423 and FTL_1422 did not have a polar effect on downstream genes, a DNA region from FTL_1421 from the parent and the mutant was subjected to RT-PCR (Fig. 6).

To address this question, each KD mutant was subjected to the binding assay.

This mutant was subjected to a second round of selection with rifabutin.

Subsequently, each seak mutant was subjected to low-resolution single nucleotide polymorphism (SNP) mapping with the BQ29 recombinant inbred strain (Wicks et al. 2001; Davis et al. 2005).

To demonstrate that YdiI is an essential catalyst for menaquinone synthesis in E. coli, the ydiI gene knockout mutant was subjected to two lines of investigation.

The oxidized S108C/L135C mutant was subjected to detailed NMR analysis to confirm that formation of the disulfide did not perturb the structure.

Cells from a chemostat culture of the HncsoS4AB Km mutant were subjected to a standard carboxysome isolation procedure [3], [15], [18] because of concerns that the orientation in which the embedded mutant cells were sectioned might have influenced the apparent shape of the carboxysomes in cell thin sections and could have led to an over-estimation of "normal" carboxysomes.

To isolate suppressors of vtc1-1, whish in in the Arabidopsis Columbia-0 background, seeds of the mutant were subjected to ethyl methanesulfonate mutagenesis.

Total RNA prepared from NIH 3T3 cells 24 h after infection by vectors holding either the wild-type Smad7 or its K359A mutant were subjected to semi-quantitative RT-PCR analyses for assessment of the transcript levels of various endogenous genes.

This pool of mutants was subjected to 20 rounds of growth, development, and spore germination in a mixed population so that cheaters that differentiate into spores with a higher efficiency would become enriched in the evolving population.

*Total number of isolated hy and gl mutants Mutation frequency = no. of mutants/no. of M2 plants × 10 To investigate the structure of mutated DNA in the isolated mutants caused by C-ion irradiation, DNA from the isolated mutants was subjected to HRM, PCR, and sequencing analyses using primers specific for the genes responsible for the hy and gl phenotypes (see Methods).