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The double mutant was transformed with pHT01ribMopt or the empty plasmid and both transformants were compared in growth assays on LB plates.
The A. oryzae adeA deletion mutant was transformed with the resulting plasmid in the presence of glucose, and the transformants were cultured in medium containing xylose as the sole carbon source.
To mimic the procedure of screening, the pET28a vector of each mutant was transformed into Escherichia coli BL21 (DE3) cells.
To investigate this possibility, a double-null mutant was transformed with a heterologous gene (mtlD) from Escherichia coli, coding for NADH-dependent mannitol-1-phosphate dehydrogenase.
In parallel, to better understand the putative functional role of VB6 and L-Trp, yfiB was deleted in a PAO1 wild-type strain, and a construct expressing the YfiBL43P mutant was transformed into the PAO1 ΔyfiB strain to trigger YfiBL43P-induced biofilm formation.
Each kinase mutant was transformed with a multi-copy plasmid expressing Adr1 from the ADH1 promoter.
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Both Col-0 plands and the atl9-1 mutant were transformed using the floral dip method [72], [73].
E. coli strains BW25113 (wild type) and JW2861-1 (Δ dsbC mutant) were transformed with pBAD33 (as control) or pBAD-AppA.
The A. thaliana plants (Col wild type or fld mutant) were transformed by the floral dip method [ 60].
Due to some genes with multiple insertions (Table 1), this pool was reduced to 12 insertions in unique loci and each of these mutants was transformed with plasmid pAJ22, which constitutively expresses the lacZ gene.
F. verticillioides fungal strain S68 (a nia-mutant) was transformed to express the GUS and nitrate reductase coding genes.
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