Sentence examples for mutant was verified from inspiring English sources
The homoplasmicity of the psbA mutant was verified by standard PCR and agarose gel electrophoresis.
The resultant mutant was verified by PCR (using primers 5'-ACAAGCGGTTTCACCCATTCGGGTTTCTACG-3' and 5'-ACAAGCGGTGTAGTTTCAGTCGTAGGCGCTG-3' anddesignated ApΔcomE1.
The ΔH28ΔH76 mutant was verified to lack superoxide dismutase (SOD) activity by its inability to complement the growth of the SOD double-negative (sodA-, sodB-) E. coli strain CK9C1891 [82].
The I130T mutant was verified by sequencing.
The mutant was verified by sequencing (BGI).
The correct insertion of the cassette on each mutant was verified by PCR.
The btcA−bteA region of the mutants was verified by DNA sequence analysis.
Each of the mutants was verified by PCR and sequence analysis.
The integrity of the RFM mutants was verified by DNA sequencing.
The entire open reading frame of all shs1-ps mutants was verified by sequencing.
The integrity of the MENT mutants was verified using: (1) far-UV circular dichroism spectroscopy (data not shown); (2) thermal stability; and (3) inhibitory activity (the association rate constant, kass, and the stoichiometry of inhibition, SI) against human cathepsin V (Table 2).
