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Exact(7)
We used the Arabidopsis 129B08/nced3 mutant rather than rice OsNCED mutant because no rice OsNCED mutant was available at the time when we initiated this study.
In order to knock-down dUTPase expression, as no dut1 mutant was available from the Arabidopsis collections of insertion lines, we produced RNAi/DUT1 lines [16].
fasn-1 RNAi phenocopied the embryonic lethal phenotype of emb-14 (g43ts ), however, no deletion mutant was available for further validation by complementation tests.
In the original manuscript, we tested all those loci recurrently mutated by our original analysis for which a deletion mutant was available.
Among the three genes, the KO mutant was available for Ncap3b (FGSC#11856, Δ Ncap3b) and we therefore investigated its lignocellulase secretion capacity (Fig. 6a, b).
It should be finally remembered that most of the experiments described here were performed with mouse NBD1, because its ΔF508 mutant was available to us while the human ΔF508 mutant was not.
Similar(53)
Unfortunately, no cxcr7 mutant is available to test directly this hypothesis.
No tyrb mutant is available at present in fish.
If a mutant is available in a gene of interest, the morphant and mutant phenotypes should be compared.
Names corresponding to mutant phenotype should be used when a mutant is available with the name describing some aspect of the corresponding phenotype.
If the wild-type progenitor of the mutant is available, this sample should be used to obtain the wild-type sequence.
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