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Any of these factors that would favour the mutant or WT strain could create an unbalance and allow one to dominate the other.

The GFP protein, either mutant or wt, was fused to a 6xHis tag at its C-terminal.

No significant differences were observed in the capacity of mutant or wt PB2 proteins to produce polymerase complexes.

Individual (rather than pooled) IR macrophage clones were infected with each mutant or WT Mtb at MOI  = 10 and fluorescence induction was measured as described above.

No differences in the response to oxidative stress, detected with SUMO2/3 antibodies, was observed between cell expressing Y372F mutant or wt ZnT3 (Fig. 3B).

Of particular interest, elevated CCL21 protein levels were detected by IHC in Chrnb2−/−mutant retinas at P4, as predicted by the microarray result, but not in adult mutant or WT retina, also consistent with the microarray result (Figure 5D).

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Similar(31)

Over 100 cortical neurons expressing either mutant or WT-HSPB8-GFP constructs were examined and no beaded or shortened neurites were observed.

Over 100 motor neurons expressing either mutant or WT-HSPB8-GFP constructs were examined and no link could be observed between TUNEL-positive cells and mutant HSPB8 expression.

Cells expressing the S279A mutant, S282D mutant, or wt-Cx43 exhibited intact synchronous Ca2+ oscillations and elevated LY uptake compared with vector-treated cells, whereas cells expressing the S282A mutant displayed uncoordinated Ca2+ spiking and decreased LY uptake.

To study the effects of wt-p53 and some p53 mutants on −670 hMMP-9 promoter activity, 1.5 million CTB per well were seeded in 12-well plates and cotransfected with 3 µg of −670 hMMP-9 promoter/ CAT reporter plasmid and 0.5 µg of mutant or wt-p53 constructs.

WT plants under these conditions accumulated 20 40% lower levels of OSU1 transcript than plants at 0.5C/0.05N (Figure 7A), which did not induce anthocyanins in either osu1 mutants or WT.

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