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Expression of the Trask M4 mutant in these cells leads to a profound inhibition of cell adhesion (Figure 5B).
Introduction of the mutant in these cells even reduces invasiveness and metastatic ability (14, 18).
However, we found that the auxin efflux (activation) of the wild type PIN1 protein was indistinguishable from the behavior of the PIN1 D215S mutant in these oocyte experiments.
Moreover, expression of wild-type USP45, but not catalytically deficient USP45 [Cys199Ala] mutant in these cells, restored the number of spontaneous ERCC1 foci almost to the level seen in wild-type U2OS cells (Fig 6A and B).
We included a PDI ΔS1S2 mutant in these assays as a control to demonstrate that any disulfide exchange observed with the wt PDI could only be due to its active site disulfide.
Similar(55)
The tool we used to generate mutants in these case studies is called Milu [33] (version 3.211).
Mutants in these genes lack either the anterior or posterior part of the segmented pattern.
We constructed insertion mutants in these two genes, presuming that they are monocistronic (Figure 6).
This hypothesis should be tested by focused mechanistic studies in single and double mutants in these genes.
The difference in the observed pathogenicity of conidiation color mutants in these different host models is most likely attributed to the difference in host immune responses induced by the color mutants.
Despite evidence that caps and trn have an important function in maintaining compartment borders in the wing, previously studied mutants in these genes have not affected the dorsal-ventral boundary [11].
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