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Exact(8)
Apart from the structural information, our biochemical analysis showed that the E144A mutant has only 5% activity remaining, with similar iron content as measured for the wild type (Fig. 4 and Table 2).
For example, the rice moc1 mutant has only the main culm with no tillers [66]; moc1 is a recessive mutation of MOC that encodes a putative GRAS family nuclear protein regulating the control of tillering.
Since it has been reported that bacterially expressed Y271A mutant has only 1% activity of wild type enzyme [9], we asked whether the mutants would similarly affect RT activity in the viral particles.
This mutant has only 2% spore viability, compared with more than 90% in wild type (Acharya et al. 2008).
The authors say that K78E and R132E are required for RAP stimulation but not essential for enzyme activity, however the double mutant has only 63 % activity.
In addition, a triple site mutant C+DE was also tested, this mutant has only one functional site, the fourth site (Willox and Royle, 2012).
Similar(51)
Combining the motif 1 mutant had only a minor additional effect (Fig. 5B).
Indeed, the pal1 pal2 pal3 pal4 quadruple mutant had only 20-25% of residual lignin [ 50].
As expected from the results shown in Fig. 6B, the cell extract from the tpm1Δ mutant had only a slightly better actin assembly rate than that of the rad2C65Δ mutant (Fig. 7B).
Similarly, we observed that the mutant had only a minor impact on the FG/FxFG2 601Nsp1 gel, but severely inhibited the entry of NTR·cargo complexes and inert objects into the GLFG gel.
On analysis of seeds per silique, the WT and single mutants bet11/− and bet12/− had a mean of 47 43 seeds per silique, whereas the bet11/−bet12/+ and bet11/+ bet12/− double mutants had only about half the amount of seeds, 20 21 (P < 0.01 by Student's t test).
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Justyna Jupowicz-Kozak
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