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Southern blot analyses showed that this mutant carries only one copy of the transposon integrated in its genome (Fig. 3A).
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However, the mir168a-2 mutant, which carries only a MIR168b locus, does not develop normally when stressed in a glasshouse, suggesting a role for MIR168a during stress adaptation.
Nevertheless, it is quite surprising that none of the mutants showed a growth defect, especially BY4741pdc2Δ519 that carries only the mutant version of Pdc2p with a deletion of 519 amino acids which accounts for 56% of the wild type protein.
The moderate reduction observed in BY4743pdc2Δ519 was within the expected, however, it is surprising we didn't observe any phenotype for the haploid BY4741pdc2Δ519, which carries only the mutant copy of the PDC2 gene.
Each parent carries only one mutant allele but all three affected children are compound heterozygous for both mutations.
Triple mutant plants carrying only CRWN2 or CRWN3 were extremely stunted, but still viable.
And Xiandaihua — modernization — carries only material meaning.
Toys RR" Us carries only a limited selection.
The pattern in met-1 mutants, which carry only MES-4-dependent H3K36 methylation activity, is strikingly different.
We characterized the behavioral and molecular phenotypes of mice carrying only the mutant allele (and thus modeling RTT) and mice carrying the mutant allele in addition to a WT allele (modeling MECP2 duplication syndrome) in an effort to determine how each mutation affects the function of the protein in the two diseases.
Hemizygous males and homozygous females with low-activity mutant forms of the enzyme carry only G6PD-deficient erythrocytes.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com