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Trp134 mutations did affect the Km values for phosphite, with the Trp134Ala mutant exhibiting a 40-fold increase in Km,PT.
CBP60g and SARD1 have partially redundant function in SA signalling with both mutants affecting SA accumulation and pathogen growth but the double mutant exhibiting a greater than additive effect [ 19, 20].
This hypothesis has been supported by the generation of the Talpid3 -/- mouse, which in many ways phenocopies the chicken mutant; exhibiting a loss of cilia due to disrupted centrosomal migration and developing aberrant Shh signaling, polydactyly, and loss of facial midline structures (Bangs et al., 2011; Davey et al., 2006; Hinchliffe and Ede, 1967; Stephen et al., 2013; Yin et al., 2009).
Further, prior demonstrations of RNAP road-blocking used lac repressor or non-cleaving EcoRI proteins that bind with Kds of 0.3 1 pM (∼10 tighter than we observe for H-NS, 2 nM), whereas only partial road-blocking was observed with an EcoRI mutant exhibiting a Kd of 30 pM (still 100× tighter than H-NS) (Deuschle et al., 1986; Pavco and Steege, 1990).
For the EmrE mutants, changing the loop length leads to relatively small shifts in the EOT ensemble; however, for the CB mutants, the effect of loop length on the EOT ensemble is much larger, with the new short-loop CB mutant exhibiting a much weaker bias towards the Ncyto/Ccyto topology in the EOT ensemble.
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The mutant exhibited a distinct yellow-green leaf phenotype throughout development.
The best mutant exhibited a threefold activity increase in the conversion of β-phenylalanine compared to the wild-type.
The mutant exhibited a distinct yellow-green leaf phenotype throughout development, reduced chlorophyll level, and arrested chloroplast development.
The crown root less5 (crl5) mutant exhibits a reduced number of crown roots due to a reduction in crown root initiation (Kitomi et al. [2011]).
Targeted Δtri3 knockout mutant exhibited a sharp decline in the production of trichodermin, and trichodermol, which is a substrate for trichodermin production, accumulated.
The SsrASTOP mutant exhibits a striking hypersensitivity to ROS.
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