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While the distinct 80 kDa and 85 kDa bands represent different post-translationally modified cortactin forms associated with pS405/418 phosphorylation, mutations at these sites have no effect on 80/85 kDa cortactin ratios, with the S405/418A mutant displaying a similar cortactin electrophoretic pattern to wild type cortactin (Figure 4).
While the wild-type exhibited only a slow increase in the percentage of dissectable body fat, all mutants accumulated fat very rapidly after 2 months of age, with the 569 mutant displaying a rate of perirenal fat accumulation that was similar to the GHR−/− mutant and slightly faster than the 391 mutant (Figure 2E).
The pfk2Δ mutant was the only mutant displaying a drop in NADH levels after glucose addition.
female fertile-1 (ff-1) was originally isolated as a mutant displaying a defect in female fertility.
Another mutant displaying a spotted pattern is seurat, encoding the transmembrane protein immunoglobulin superfamily member 11 (Igsf11) (Eom et al., 2012).
The mutant, displaying a nonacidifying phenotype, was crossed to A. niger strain N879 and a nonacidifying pyrG-, prtT- segregant (D15 26) was selected for further studies.
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The DAAO coupled assay is sensitive in that it allowed the detection of a DAAT mutant displaying an approximately 2000-fold decrease in kcat/KM relative to wild type.
The C. jejuni Δppk2 mutant displayed a significant dose-dependent colonization defect in chickens (Figure 8).
As expected, the vpsR mutant displayed a low isosurface volume of biofilm.
The K14/A mutant displayed a similar fibril morphology to the ΔK14 mutant, albeit the fibrils were thinner (5.5 to 8.5 nm) and fewer (Figure 9A).
The HPr-S46A mutant displayed a 400-minute extension of the ripening period, whereas HPr-S46D mutant displayed a moderate extension of 100 minutes (Fig. 6a, c).
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com