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H209N was the most thermostable mutant, showing a Tm of 71.3 °C.
One such mutant showing a Smr Kmr phenotype was selected for further study and designated CHA6.8 (Table 1).
The mutant G114C/WT formed similar UV-vis spectra to E113C/Basal mutant, showing a pigment in light absorption (λmax = 380 nm) (Figure 2).
Only 10 20 flat cells were found in cultures transfected with the RB-KN mutant, showing a statistically significant reduction in the flat-cell induction activity of this mutant (Fig. 3B).
The only mutant showing a significantly greater number of phagocytosed RBC than the parental strain was EPECΔ espJ (strain ICC190) (Dahan et al., 2005).
The highest autoxidation rate is observed for the F28H mutant, showing a 50-fold50-foldse versus that of the WT (Table 1).
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The best mutant showed a 4.3 °C increase in its T5015.
The triple mutant showed a loss of ubiquitin ligase activity in vitro and in cells (Fig. 7).
The Glu56Ser (E56S) mutant showed a 1.6-fold higher activity than the wild-type enzyme.
On the other hand, the 1E/Q mutant exhibited an increased ATPase activity, while the 2E/Q mutant showed a slightly decreased ATPase activity (Supplementary Figure 14a).
As compared with ΔN-TClpB, the 2KT/AA mutant showed a significantly broader distribution with a center value of 42.1 nm and a width of 9.0 nm.
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