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Exact(4)

For cul1, cul3 and slmb mutant clones, data are presented as means±SD.

The specificity of Spastin staining was further confirmed by the absence of the staining in the spastin5.75 [10] protein-null mutant clones (data not shown).

No difference was found between the hFXN and mutant clones (data not shown), indicating that these cells did not loose their mitochondrial DNA.

In contrast to the effects of P35, if JNK signalling was blocked in scrib mutant cells expressing aPKCCAAXDN, by co-expressing BskDN, not only was cell viability dramatically restored but the mutant tissue also exhibited normal morphology, although occasional scaring and basally located photoreceptor cell nuclei were still sometimes observed at the edges of mutant clones (data not shown).

Similar(56)

A similar result was seen with dor, dsh, stbm triple-mutant clones (data not shown).

The more novel observation of autonomous 10xStat>GFP activation within ept,H99 mutant clones agrees with the data gathered with the pY-Stat92E and 3xGAS-lacZ reporters.

Finally, we note that we have not been able to detect misexpression of nubbin, homothorax or vestigial in either ph or Psc-Su z 2 Psc-Su z 2nes in wing discs (data not shown).

Furthermore, using a probe to the semiconserved var exon II, transcripts of around 9 kb were identified in ring-stage RNA of FCR3-CD36 and in the mutant clone 1F1-HA (data not shown).

However, no definite conclusions can be drawn, as the expression data and binding data originate from different mutant clones.

Similarly, we find that cad, Dll and wg become misexpressed in PcG mutant clones in imaginal discs (Beuchle et al, 2001, and data not shown).

No differences in cell size were observed in Asx mutant clones in larval or pupal wing discs (Figures 3,5), and data not shown, and the number of cells within mutant clones was comparable to wild-type at these stages.

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