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Nevertheless, no significant spindle mis-positioning was observed in these tub4 mutant cells (data not shown).
Our extensive analyses fail to reveal a significant difference between the WT and mutant cells (Data not shown).
However, a growth advantage was not observed in ctf7-203 elg1 rad24 triple mutant cells, relative to ctf7-203 elg1 double mutant cells (data not shown).
The basal markers Integrin-βPS and Talin were also localized throughout the membrane in the expulsed ph0 mutant cells (data not shown).
TEM examination of macrophages directly after phagocytosis did not show any significant difference in the intracellular distribution of wild-type or hla mutant cells (data not shown).
This low level of intracellular MF201 Legionellae could not be explained by a defect in host adherence because no difference was observed between the numbers of non-adherent parental or mutant cells (data not shown).
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Induction of ULBP1 transcription by the proteasome inhibitor MG132 (Butler et al., 2009) was not prevented in the ATF4-mutant cells (data not shown), indicating that certain cellular perturbations transcriptionally activate ULBP1 independently of ATF4.
Since production of the ΔN2-PINK1 fragment is unaffected in our PINK1 cleavage mutant cells, our data demonstrate that the ΔN2-PINK1 compensatepensate for the loss of ΔN-PINK1 because mitochondrial function is still impaired in its presence.
However, real time PCR analyses showed that Pax2 RNA declines three fold in mutant green cells (data not shown).
Also increasing dose of Doxorubicin did not induce endogenous SMAR1 expression in p53 null as well as mutant cell lines (data not shown).
Analysis of HAP1 cell lysates by Western blot confirmed the loss-of-protein expression in ATF4, RBM4, and SPCS2 mutant cell lines (data not shown).
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