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High expression of methyltransferase type 12 domain containing protein and PPi-PFK performed by qRT-PCR analysis in rad mutant at both 1 and 3 days after germination compared to wild type supports their involvement in cell survival (Fig. 1).

Many fewer WT bacteria were associated with PMNs than either ΔdltA mutant at both time points tested (Fig. 3).

The NWMN_1655 gene, which encodes the virulence factor regulator protein Rot, was expressed at decreased levels (about 3-fold) in the ecsA::intron mutant at both growth phases.

After successfully knocking out the ssrA gene using the cosmid-mediated recombination methodology, we were able to demonstrate germination, growth, and sporulation defects of ΔssrA mutant at both high and routine growth temperatures.

The HapR levels were increased in the ΔrpoN mutant at both OD 1.0 and 2.0 (Fig. 7C), in agreement with the previous reports that the hapR gene expression is negatively regulated by the RpoN sigma factor through four small RNAs, Qrrs, and Hfq in the quorum-sensing regulatory pathway of V. cholerae [27], [28].

However, we observed increased petite frequency in the erg26 mutant at both the permissive and semi-permissive temperatures and that this phenotype can be suppressed by HU.

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We found that the overall distribution of the GFP-Eps15 in transfected cells is the same for wild type and TS-mutant at both permissive and non-permissive temperatures (figure 8C) at the plasma membrane and TGN.

We also detected significantly increased proliferation of non-cardiomyocytes in mutants at both week 6 and week 16 (red cells in Figure 4A–B').

At the FBA1 gene, changes were observed in the npl3-S411A and rna15-2 mutatts at both ends of the gene denoting a terminating effect in the flanking MPE1 gene (Figure 5A).

The proportion of this defect was 90% in average for the mutants at both temperatures and 6% (22°C) and 11% (34°C) in wild-type teliospores (Fig. 10C and not shown).

To better measure p53 target gene expression levels, I performed quantitative polymerase chain reaction (qPCR) analyses on shh−/− mutant versus wild-type embryos at 24 hpf and 56 hpf confirming elevated expression of p53, p21 mdm2, cycG1, bax1 and puma in shh−/− mutants at both stages (Fig. 1D, E).

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