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The endogenous Unc45b in muscle cell lysates is also found associated with Hsp90.
The endogenous Unc45b in muscle cell lysates also interacts with Hsp90 and isolates as a complex with the chaperone.
In order to assess whether PDN treatment of C2C12 myotubes attenuates global translation we fractionated muscle cell lysates by sucrose density gradient centrifugation.
The α6 chain content was evaluated in the same muscle cell lysates.
To investigate the potential role of BNip3, we analyzed its expression levels in muscle cell lysates from four HD and four control subjects by SDS-PAGE.
Though we could not directly compare levels of aggregated and filamentous myosin (both species contribute to the insoluble fraction of muscle cell lysates), our interpretation is corroborated by recent publications reporting the reduced stability and aggregation of unassembled muscle myosin (Kurapati et al., 2012 ; Martinsson et al., 2000 ; Tajsharghi and Oldfors, 2012 ; Tajsharghi et al., 2005 ).
Similar(53)
Furthermore, Western blotting of whole muscle cell lysate showed that the antibody gave a band of the approximately 500 kDa corresponding to the size of plectin (data not shown).
The one characterized by a slightly higher molecular weight virtually disappears in the PD-treated groups (Fig. 5B) as well as in PD-untreated TB muscles when cell lysates are incubated in the presence of phosphatase (Fig. S4).
We immobilized WT and mutant (M712T) GNE proteins to the BIAcore sensor chip, and run through it anion exchanged fractions of human skeletal muscle primary culture cell lysate.
Activity of PADs has been demonstrated in various cell lysates, such as muscle, ovary and spinal cord [ 14].
Total cell lysates from human skeletal muscle myoblasts and HSMMs were subjected to immunoblotting.
Related(15)
muscle tissue lysates
muscle cell membranes
muscle cell relaxes
muscle cell filopodia
muscle cell phenotypes
muscle protein lysates
muscle infant lysates
muscle cell densities
muscle cell microenvironments
muscle cell cultures
muscle cell lineages
muscle cell layers
muscle cell types
muscle cell markers
muscle cell lines
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