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Through regulation of su fu), miR-214 enables precise specification of muscle cell types by sharpening cellular responses to Hedgehog.
To study the impact of myokines secreted by different muscle cell types on beta-cells, we treated rat sorted beta-cells and human islets with conditioned medium from control and TNF-alpha treated MC-S and MC-T primary cultures.
Nine of these fibrillar collagens were expressed specifically in all differentiated muscle cell types, colF-11 was highly expressed in pharynx muscle, and colF-10 had restricted expression to body wall muscle (Supplementary Fig. 4 12.
This position suggests that neural and muscle cell types were either lost in major animal lineages (e.g., Porifera) or that they evolved independently in the ctenophore lineage.
miR-214 has been implicated in specification of muscle cell types by modulation of Hedgehog signalling [54].
Recently, we identified a subpopulation of human circulating stem cells expressing the CD133 antigen that can differentiate into endothelial and muscle cell types [16].
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We do not exclude other possible myokine secretions to be muscle cell type dependent as we have used here a targeted approach to define different myokines groups.
Inhibition of miR-214 results in a reduction or loss of slow-muscle cell types.
To further our understanding of FSHD and normal myogenesis, the expression profiles obtained were compared to those of 19 non-muscle cell types analyzed by identical methods.
For example, all four Argonaute genes involved in RNA-silencing were significantly upregulated during normal (but not FSHD) myogenesis relative to non-muscle cell types.
There is a significant literature on the ability of p50 homodimers to induce transactivation when bound to Bcl-3 in several non-muscle cell types [21], [22], [23].
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