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Vascular endothelial and smooth muscle cell phenotypes may change dramatically after isolation and in cell cultures.
This study was designed to investigate the relevance between smooth muscle cell phenotypes and extracellular matrix disorders in the dissected media.
Lipid and muscle cell phenotypes were characterized as described above.
In our prior work on SMCs grown on multilayers, we relied on patterns of stained actin filaments and measurements of motility to distinguish smooth muscle cell phenotypes.
These changes in composition and properties may contribute to endothelial cell loss and dysfunction, altered vascular smooth muscle cell phenotypes and a decrease in the mechanical stability of the lesion cap, thereby increasing the propensity of lesions to undergo rupture.
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Immunocytochemistry was used to define smooth muscle cell phenotype, and c-fos expression levels were assessed by Northern blotting.
Stromal cell cultures were established from surgical specimens and the differentiated smooth muscle cell phenotype was monitored by gene expression, immunofluorescence and immunoblotting.
Transforming growth factor-β1 is thought to have a role in bladder pathology by modulating the bladder smooth muscle cell phenotype and, thus, interactions with the extracellular matrix.
Our data revealed the potential to upregulate matured vascular smooth muscle cell phenotype (myosin heavy chain expression) of hMSCs by employing appropriate matrix stiffness and growth factor (within 72 h).
Stromal cells were seeded onto plasma-coated electrospun polystyrene scaffolds to examine the influence of 3D architecture on smooth muscle cell phenotype, but differentiation was inhibited by serum proteins that adsorbed non-specifically onto the large surface area of the scaffold.
Thus, increased SOD-2 may play a crucial role in determining plaque phenotype as it directly affects smooth muscle cell phenotype.
More suggestions(15)
muscle morphology phenotypes
muscle maturity phenotypes
muscle cell densities
muscle detachment phenotypes
muscle cell microenvironments
muscle cell lysates
muscle strength phenotypes
muscle hypertrophy phenotypes
muscle cell cultures
muscle atrophy phenotypes
muscle arm phenotypes
muscle fiber phenotypes
muscle cell types
muscle cell lineages
muscle cell precursors
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