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HepG2 (human hepatoma cell line), fibroblasts (L929, murine fibroblast line), and other cell lines exhibit strong adhesion on areas of UV-irradiated polymer.

For these experiments, we developed a cytotoxicity assay using a murine fibroblast line (NIH 3T3 cells) which endogenously expresses CD1d and can present α-GalCer to activate human NKT cells in a dose-dependent fashion, as measured by up-regulation of the IL-2 receptor α chain, CD25 (Figure 5A).

Cells of the murine fibroblast line 3T3NIH were transfected with retroviral vector DFG-hIL-1β-neo, which contains the cDNAs for human (h IL-1β and neomycin phosphotransferase (neoR), as described previously [ 17].

The favorable growth kinetics in low initial B cell seeding density between the soluble formulations and the CD40L-expressing murine fibroblast line likely reflects the difference in CD40L bioavailability when provided as soluble recombinant form or as a continuously renewing membrane source from a cell line.

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The cytotoxic potential of the lapachones evaluated was also assayed using non-tumor cells: human peripheral blood mononuclear cells, two murine fibroblast lines (L929 and V79 cells) and MDCK (canine kidney epithelial cells).

The cell lines used were the murine neuroblastoma cell line N2a-PK1 [22], the murine fibroblast cell line NIH3T3 [23], and the Npl and Hpl3 4 cell lines derived from Prnp-ablated mice [24], [25].

However, Penning et al (1994) showed that the inhibition of a DNA repair enzyme (poly(ADP-ribose polymerase) ADP-ribose polymeraseactivity irreversible DNandamage were correlathe to the killing eformationhematoforphyrirreversiblee PDNAin a murine fibroblast cell line; note that Photofrin is purifiedamage of hematoporphyrin derivative.

The cell lineages HEK293T (human embryonic kidney cell line) [ 27], NIH3T3 (murine fibroblast cell line) [ 28], and H9C2 (rat cardiomyoblast cell line) [ 29] were maintained in Dulbecco's Modified Eagle Medium (DMEM, GIBCO, Auckland, New Zealand) supplemented with 10% fetal bovine serum (FBS, GIBCO), 0.2 mM glutamine (Glutamax-1, GIBCO), 10 U/mL penicillin, and 10  μg/mL streptomycin (GIBCO).

The human breast cancer cell line MCF-7 was purchased from American Type Culture Collection (ATCC) and murine fibroblast cell line NIH/3T3 was purchased from the Cell Bank of the Federal University of Rio de Janeiro Rioo de Janeiro, Brazil).

The NIH-3T3 murine fibroblast cell line [German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany)] and the human lung epithelial cell line A549 (ATCC #CCL-185) were maintained in DMEM containing 10% FCS and cultured in a humidified atmosphere of 5% CO2 at 37°C.

Plasmids pEPito-CMV-EGFPLuc, pEPito-AFP-EGFPLuc and pEPito-hCMV/AFP-EGFPLuc were transfected into 13 different human and murine cancer cell lines (hepatoma, cervix carcinoma, colon carcinoma, glioma, prostate carcinoma, melanoma, squamous cell carcinoma, neuroblastoma) and a murine fibroblast cell line.

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