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TRPV1-NIH3T3 mouse fibroblast line, ectopically expressing the rat TRPV1 was established previously.
In addition, a mutant mouse fibroblast line (GD25) lacking β1 integrin showed significantly reduced bacterial invasion [ 23].
These cells are a mouse fibroblast line that stably express a Gli-dependent firefly luciferase and a TK Renilla luciferase control to allow quantitation of Hh pathway activation.
A similar effect was observed in HeLa cells (Fig. 1F and G) and in NIH3T3 cells, a mouse fibroblast line (Fig. 1H and I).
Mouse fibroblast line 3T3-Ll preadipocytes were maintained in DMEM, 50 U/ml penicillin and 50 μg/ml streptomycin, 3.7 mg/ml NaHCO3, and 10% CS, with a replacement of media every other day.
As a model system, we chose a mouse fibroblast line for which biophysical data included cell speed, membrane protrusion activity, cell spread area, surface adhesion, and membrane retraction (previously measured by Maheshwari et al [ 10]).
Similar(53)
In recent studies we have analyzed the distinct effects of different embryonic mouse fibroblast lines on epidermal morphogenesis of human keratinocytes, thus establishing a sensitive and efficient read out system for fibroblast derived mediators (10, 16, 25).
We used wild-type and Jnk1/Jnk2 double knockout mouse fibroblast lines to show that Jnk and p38 are involved in the phosphorylation of B-Myb in UV-stressed cells.
It has been recently reported that, in addition to -carnitine, its acetylated derivative is also able to reduce apoptosis in primary neuron cultures and in stable mouse fibroblast lines.
Mutations at amino-acid 482 have included R482G and R482T in human cancer cells; R482S and R482M in mouse fibroblast lines (Honjo et al, 2001; Allen et al, 2002); and a recently reported R482M mutation in a doxorubicin-selected human T-cell line (Wang et al, 2003).
In an early study (Minoura et al. 1995b), BMSS harvested directly from the silk gland was evaluated as a substratum for mouse fibroblasts (line L-929), alone or blended with fibroin.
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