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Several of the TERC mutations have previously been assayed for telomerase activity in an in vitro fibroblast line system using co-transfection of plasmids bearing TERT and TERC with the mutations in question and these results have been noted (Table I).
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For this, we used Oli-neu cells, an immortalized cell line capable of differentiating into immature oligodendrocyte-like cells in vitro [ 15], and COS7 cells, an immortalized fibroblast line.
Interestingly, in vitro fibroblast models with p53 mutations have been found to be hypersensitive to paclitaxel but resistant to platinum, while cell lines with normal p53 function are most sensitive to platinum (Wahl et al, 1996).
293T (EBV−,KSHV−) is human fibroblast line.
To set up an in vitro cellular model of HGPS, we generated iPSCs from two HGPS primary skin fibroblast lines (HGADFN164: HGPS-1 and HGADFN155: HGPS-2, respectively) and one age-matched normal fibroblast line (AG08470) by retroviral transduction of KLF4, SOX2, OCT4, and C-MYC cocktails [ 27, 28] (See table S1 for cell line information).
Patient fibroblast lines used: 1133, 1134.
BCL2 blocks apoptosis in many diverse cancers, and in-vitro work using a rodent fibroblast cell line, suggests that MDV Meq increases BCL2 mRNA [ 46], and proposed that this is important in MD lymphomagenesis.
We treated a normal fibroblast cell line in vitro with shock waves under different conditions of impulses and energy.
Secreted pEGF was a fully biologically active protein, as demonstrated by its capacity to stimulate L929 mouse fibroblast cell line proliferation in vitro.
The study was performed in two human neoplastic cell lines (HeLa, A549), and human fetal lung fibroblast cell line (MRC-5), which was used as noncancerous model for in vitro toxicity evaluation.
A fibroblast cell line (208F) was used for in vitro evaluation of surfaces coated with 45S5 Bioglass®.
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