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DistMap is another method for distributed sequence alignment [ 22], which is cloud-based and can use multiple sequence aligners.
For multiple sequence protein alignment users can choose one out of three popular multiple sequence aligners: MUSCLE [ 27], PRANK [ 28] and MAFFT [ 29].
How about genome assemblers, FASTQ preprocessors or multiple sequence aligners?
For more than a decade, multiple sequence aligners have been optimized for their capacity to restitute structure-based MSAs.
Multiple sequence aligners use evolutionary information that is provided by the guide-tree, a cladogram that dictates in which order the sequences are initially aligned to each other.
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The sequence information was used to construct multiple sequence alignment using TARGeT-based multiple sequence aligner.
These sequences were aligned using the MUSCLE multiple sequence aligner program.
This general-purpose multiple sequence aligner is able to handle whole-genome alignments and has the advantage that it identifies syntenic blocks despite rearrangements and reversals.
Then we aligned the gene models for each file using the multiple sequence aligner MAFFT [ 53] and the phylogenetic analysis was performed with PAML [ 31].
The resulting de novo contigs were aligned against the draft sequence in the Blast's bl2seq multiple sequence aligner.
The resulting Verrucomicrobial contigs were ordered in Mauve 2.3.1 multiple sequence aligner (Darling et al. 2010) using the abundant strain as a reference to enable an analysis of gene order differences.
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