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Multiple sequence files can be loaded and analysed together.
The BLAST tool accepts either single or multiple sequence files in a FASTA format.
Aligned multiple sequence files were imported into Modeltest ver 3.7 [ 63] and the optimal model of nucleotide substitution was selected using the AIC criterion (see Additional file 3).
The input files (reference database or query set) are FastA formatted multiple sequence files and do not need to be aligned.
If multiple sequences were available for a given species and gene after respective steps [a.I to a.III] and [b.I to b.III], we chose the longest sequence from the unaligned multiple sequence files [a.IV and b.IV].
In these instances, the script multiple_sequence_splitter uses information from the corresponding GenBank file to split sequences into fragments that correspond to single genes; that is, it creates multiple sequence files of single genes [b.II].
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The set of data was exported as a multiple sequence file (MSF) format and used to build a phylogenetic tree with the MEGA3 software [ 61, 62].
Due to large genetic divergence between FIV from different species, alignment for coding regions of FIV used the program REVTRANS ver 1.4 [ 29] which takes a multiple sequence file, translates that file into amino acid residues, aligns the amino acids, and uses this alignment as the scaffold for nucleotide alignment.
The human, dog (canFam2), mouse (mm8), and rat (rn4) sequences were extracted and multiple sequence alignments files corresponding to the same RefSeq were stitched together with Galaxy.
For each RefSeq, the 28-way multiple sequence alignments files for the 3'-UTR or coding sequences were retrieved from UCSC genome table browser.
Multiple sequence alignment files are available upon request.
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