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Another advantage is that multiple replicates are available with identical conditions for each new experiment.
Grapevine, being a woody perennial crop, is challenging to use in controlled conditions experiments, especially where large amounts of material and multiple replicates are required.
Technical replicates are used to establish experimental variability in NGS procedure (Robasky et al., 2013), though multiple replicates are not necessary for RVD2.
Joint likelihoods for multiple replicates are obtained by assuming that the replicates are independent conditional on the genotypes of all contributors and parameters ϕ such as the amounts and degradation levels of DNA from each contributor [2].
FDM, on the other hand, did not predict any DP genes with this test, which was the result of the highly conservative permutation test it uses when multiple replicates are provided.
Our study confirmed all detected QTLs from this large study, which indicates that biparental populations, when using sufficient number of seedlings and multiple replicates, are not underpowered compared with studies using multiple populations.
Similar(54)
Amplification, probe preparation and hybridization protocols were performed as previously described [9] and multiple replicates were examined in each condition.
Multiple replicates were compiled for statistical analysis and presentation.
Where raw data of multiple replicates were available to us [ 12], noise variance was quantified directly.
At the time we sequenced the transcriptomes, costs associated with sequencing multiple replicates were prohibitive.
The model for data originating from multiple replicates is illustrated in Figure 3.
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