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Indeed, multiple nuclear markers have been significantly associated and genetically determined as indicators of physical performance and muscular speed [ 10].
Six of the eight analyzed populations are highly distinct based on both mtDNA and multiple nuclear markers.
We contend that the incongruence between the mitochondrial phylogeny and multiple nuclear markers, phenotypes and behavior is best explained by ancient introgressive hybridization events.
The use of multiple nuclear markers plus the independently evolving mitochondrial genome also allows further examination of the histories of range expanding populations, such as the extent to which invading populations from different origins maintain their genetic distinctiveness, or the role of sex-biased dispersal [ 17, 18].
In this study we have provided a proof of principle of this approach for meiofaunal tardigrades, an important group of soil biota previously not considered amenable to such studies, thereby paving the way for more comprehensive phylogenetic studies using multiple nuclear markers, and population genetic studies.
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The motivation behind studying independent gene trees (i.e. multiple nuclear genetic markers) is that these should ultimately converge upon the overarching species tree [ 8].
We employed multiple chloroplast and nuclear markers to investigate the evolution and diversification of Panax.
The tree topology inferred from the Bayesian analysis of the combined data agrees well with previous estimates based on multiple nuclear and mitochondrial markers.
Table 3 ANA-reflex test procedure with patterns related to markers found in autoimmune liver diseases ANA-IIF pattern on HEp-2 cells Reflex test(s) Cytoplasmic reticular/AMA ≥1 160 Anti-mitochondrial M2 or E3 or MIT3 Multiple nuclear dots ≥1 160 Anti-Sp100 Nuclear envelope Anti-gp210 Cytoplasmic linear-actin ≥1 160 IIF on kidney, stomach, and liver, to confirm anti-actin antibodies.
In the Branchiopoda, when multiple types of evidence are available (ecological and/or nuclear markers to complement mitochondrial DNA), divergences of >5% are typically indicative of different species [24], [28].
The nuclear markers adopted in the present study, ITS and 28S regions, have multiple copies in the nuclear genome.
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