Sentence examples for multiple mutations were from inspiring English sources

The LT cleavage sites for MKKs have been identified [25] and single or multiple mutations were introduced into wildtype MKKs to destroy these sites.

As had been determined previously for other proteins [14], [15], multiple tryptophan to phenylalanine replacements caused a serious reduction in stability of the variants (data not shown) and therefore variants containing multiple mutations were not analysed further in this study.

In the 5% dilution of DLD1, multiple mutations were not detected in both runs.

Multiple mutations were detected both within and beyond the QRDRs of parC and parE genes.

Genotypes of strains carrying multiple mutations were confirmed by PCR for deletions or sequencing of individual mutations.

Strains carrying multiple mutations were generated by standard methods and confirmed by polymerase chain reaction (PCR) and/or sequencing.

An additional driver gene mutation increases a cell's fitness by a factor of (1 +s d ), whereas an additional housekeeper gene mutation decreases fitness by a factor of (1 −s h ) and the effect of multiple mutations is multiplicative.

How is change possible where multiple mutations are necessary but intermediate steps have no apparent advantage?

For example, in cavefish, we do not know what is the precise mutation that causes a change in gene expression level, or if multiple mutations are involved.

In this study, the bacterial laccase gene (cueO) with single or multiple mutations was directly cloned based on the metagenome of a chemical plant sludge.

However, multiple mutations are often deleterious for the activity and the identification of all the residues that historically led to the function is complicated.