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Multiple accessions were included for each species.
In some cases for which multiple accessions were analysed per genus, the genera were recovered as monophyletic, such as Malephora N.E.Br.
While a large proportion of the available herbarium material yielded poor DNA, sequences from multiple accessions were nevertheless obtained for all species except M. ericifolia from East Africa (represented by a single accession).
For those species for which multiple accessions were included, sequences for all accessions fell into the same clade, though in several cases accessions from multiple species grouped together with strong support (e.g. D. clintoniana and D. cristata).
However, in the majority of cases where multiple accessions were sequenced, accessions Acrodon N.E.Br., Delosperma N.E.Br., Faucaria Schwantes, Lampranthus N.E.Br., Oscularia Schwantes and Ruschia Schwantes were distributed across core Ruschioideae, highlighting the lack of resolution or, alternatively, providing evidence for non-monophyly of these genera.
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For each species multiple accessions are included.
Fourthly, it could indicate that taxa whose multiple accessions are appearing in diverse clades represent cryptic species, where broad morphological concepts on species are masking genetic patterns.
The branching pattern is consistent with the recovery of a single β-amylase homolog, in that most of the genera from which multiple accessions have been sampled form monophyletic groups (Secale, Australopyrum, Dasypyrum, Hordeum, Pseudoroegneria, and Taeniatherum); this pattern is indicative of orthology.
Although most of these accessions showed partial membership in multiple clusters, 50 out of the 80 accessions were assigned to the same cluster as other accessions originating from the same population.
Multiple accessions can be queried by entering accession numbers separated by a single space character.
Multiple accessions can be searched by entering accession numbers separated by a space.
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