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The lcc1 gene coding for the laccase from Trametes versicolor DSM11269 was cloned into the genome of Yarrowia lipolytica using either single or multiple integration sites.
Multiple integration sites within a gene were also detected in 11 16% of tagged genes in DNA samples from fish that were injected with SB11 mRNA.
When the female line was bred, a high level of transmission (~90%) was observed, indicating multiple integration sites of the transgene.
Differences in chromosomal location have been avoided by integrating different constructs at the same locus (16) or mitigated by averaging the behaviour of constructs at multiple integration sites (23).
These data support the feasibility of chromosomal expression of a foreign antigen from multiple integration sites, and the possibility of antigen expression synchronized with growth-rate, a possibility not supported by plasmid-based expression in these experiments.
The pervasive expression of GFP in P0 individuals could be due to the early integration of the transgene during development and/or to multiple integration sites of the transgene in the genome.
Similar(54)
Use of DNA bar coding allowed multiple integration site populations to be studied in parallel [47] [49].
Because of its flexibility, the multiple integration site of mobile DNA can be considered an integration hotspot (IHS) that increases the genomic plasticity of the bacterium.
Nevertheless, Demircan [ 36] suggests that there could be multiple transgene integration sites, which are, however, expected to be located close to each other in the genome.
ICEs also include conjugative transposons, a terminology that had been used mostly for elements that originated in gram-positive bacteria, and, according to some authors, should be reserved for elements, which can target multiple different integration sites (Burrus et al., 2002).
Multiple XMRV chromosomal integration sites were found in the 22Rv1 cell line as well as in that of cancer tissues of PC patients [4].
Related(14)
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multiple entry sites
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multiple integration cassettes
multiple integration options
multiple integration techniques
multiple infection sites
multiple binding sites
multiple insertion sites
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